Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Samuel, J.; Tanner, M.E.
    Active site mutants of the "non-hydrolyzing" UDP-N-acetylglucosamine 2-epimerase from Escherichia coli (2004), Biochim. Biophys. Acta, 1700, 85-91.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.1.3.14 UDP-N-acetyl-D-glucosamine allosteric activation Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.14 wild-type and mutant enzymes expressed in Escherichia coli Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.14 D95N about 18000 fold decrease in turnover number for UDP-N-acetyl-D-glucosamine, not possible to obtain accurate kinetic constants Escherichia coli
5.1.3.14 E117Q about 18000 fold decrease in turnover number for UDP-N-acetyl-D-glucosamine, not possible to obtain accurate kinetic constants Escherichia coli
5.1.3.14 E131Q about 18000 fold decrease in turnover number for UDP-N-acetyl-D-glucosamine, not possible to obtain accurate kinetic constants Escherichia coli
5.1.3.14 H213N 30fold increase in Km-value and 50fold decrease in turnover-number for UDP-N-acetyl-D-glucosamine. Unlike the wild-type enzyme no inhibition is detected at UDP-concentrations up to 10 mM Escherichia coli
5.1.3.14 K15A more than 100fold increase in KM-value for UDP-N-acetyl-D-glucosamine Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.14 0.6
-
 UDP-N-acetyl-D-glucosamine 37°C, pH 8.8, wild-type enzyme Escherichia coli
5.1.3.14 17.2
-
 UDP-N-acetyl-D-glucosamine 37°C, pH 8.8, muatnt enzyme H213N Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.14 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.14 recombinant wild-type and mutant enzymes, His-tagged proteins Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.14 UDP-N-acetyl-D-glucosamine
-
 Escherichia coli UDP-N-acetyl-D-mannosamine
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.1.3.14 non-hydrolyzing UDP-N-acetylglucosamine 2-epimerase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.14 0.15
-
 UDP-N-acetyl-D-glucosamine 37°C, pH 8.8, muatnt enzyme H213N Escherichia coli
5.1.3.14 7.1
-
 UDP-N-acetyl-D-glucosamine 37°C, pH 8.8, wild-type enzyme Escherichia coli